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  Bolan, who is also Donahue's great-nephew, said Donahue
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  All appeared to be in their 20s. Only one, a woman from El Salvador who refused to talk to officers, was taken into custody, because she had an immigration detainer.
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  In certain embodiments, contemplated herein is the use of fluorescence polarization assays and
  fluorescence resonance energy transfer (FRET) assays in measuring, either directly or indirectly, the degree of
  interaction between an ActRIIA polypeptide and its binding protein. The bound compounds may be detected usually using colorimetric or fluorescence or surface plasmon resonance.
  Optionally, these compounds can further be tested in animal models to assess their ability to
  modulate tissue growth in vivo. Alternatively, the gene
  encoding an activin or ActRIIA polypeptide can be transfected
  with a reporter system (e.g., .beta.-galactosidase, luciferase, or green fluorescent protein) into a cell and screened against
  the library preferably by a high throughput screening
  or with individual members of the library. In some embodiments
  anemia response is measured as it relates to increased red blood cell (RBC)-transfusion independence, e.g., proportion of subjects who become RBC-transfusion free over
  any consecutive 84-day period (e.g., in subjects receiving luspatercept).
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